Genetic characteristics and ploidy trigger the high inducibility of double haploid (DH) inducer in Brassica napus.

ABSTRACT:

Background

Our recently reported doubled haploid (DH) induction lines e.g., Y3380 and Y3560 are allo-octoploid (AAAACCCC, 2n = 8× ≈ 76), which can induce the maternal parent to produce DH individuals. Whether this induction process is related to the production of aneuploid gametes form male parent and genetic characteristics of the male parent has not been reported yet.

Results

Somatic chromosome counts of DH inducer parents, female wax-less parent (W1A) and their F₁ hybrid individuals revealed the reliability of flow cytometry analysis. Y3560 has normal chromosome behavior in metaphase I and anaphase I, but chromosome division was not synchronized in the tetrad period. Individual phenotypic identification and flow cytometric fluorescence measurement of F₁

SUBMITTER: Luo X 

PROVIDER: S-EPMC8594162 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature