The construction and analysis of ceRNA network and patterns of immune infiltration in lung adenocarcinoma.

ABSTRACT:

Background

Competitive Endogenous RNA (ceRNA) may be closely associated with tumor progression. However, studies on ceRNAs and immune cells in LUAD are scarce.

Method

The profiles of gene expression and clinical data of LUAD patients were extracted from the TCGA database. Bioinformatics methods were used to evaluate differentially-expressed genes (DEGs) and to form a ceRNA network. Preliminary verification of clinical specimens was utilized to detect the expressions of key biomarkers at the tissues. Cox and Lasso regressions were used to identify key genes, and prognosis prediction nomograms were formed. The mRNA levels of 9 genes in the risk score model in independent clinical LUAD samples were detected by qRT-PCR. The interconnection between the risk of cancer and immune c

SUBMITTER: Li J 

PROVIDER: S-EPMC8594182 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature