Metabolic Remodeling during Nitrogen Fixation in Zymomonas mobilis.

ABSTRACT: Zymomonas mobilis is an ethanologenic bacterium currently being developed for production of advanced biofuels. Recent studies have shown that Z. mobilis can fix dinitrogen gas (N₂) as a sole nitrogen source. During N₂ fixation, Z. mobilis exhibits increased biomass-specific rates of ethanol production. In order to better understand the physiology of Z. mobilis during N₂ fixation and during changes in ammonium (NH₄⁺) availability, we performed liquid chromatography-mass spectrometry (LC-MS)-based targeted metabolomics and shotgun proteomics under three regimes of nitrogen availability: continuous N₂ fixation, gradual NH₄⁺ depletion, and acute NH₄⁺ addition to N₂-fixing ce

SUBMITTER: Martien JI 

PROVIDER: S-EPMC8594446 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature