Ontology highlight
ABSTRACT:
SUBMITTER: Trainor BM
PROVIDER: S-EPMC8594471 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Trainor Brandon M BM Pestov Dimitri G DG Shcherbik Natalia N
RNA (New York, N.Y.) 20210827 12
Stress-induced molecular damage to ribosomes can impact protein synthesis in cells, but cell-based assays do not provide a clear way to distinguish the effects of ribosome damage from stress responses and damage to other parts of the translation machinery. Here we describe a detailed protocol for the separation of yeast ribosomes from other translational machinery constituents, followed by reconstitution of the translation mixture in vitro. This technique, which we refer to as ribosome separatio ...[more]