Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon protease.

ABSTRACT: The FinO-domain protein ProQ belongs to a widespread family of RNA-binding proteins (RBPs) involved in gene regulation in bacterial chromosomes and mobile elements. While the cellular RNA targets of ProQ have been established in diverse bacteria, the functionally crucial ProQ residues remain to be identified under physiological conditions. Following our discovery that ProQ deficiency alleviates growth suppression of Salmonella with succinate as the sole carbon source, an experimental evolution approach was devised to exploit this phenotype. By coupling mutational scanning with loss-of-function selection, we identified multiple ProQ residues in both the amino-terminal FinO domain and the variable carboxy-terminal region that are required for ProQ activity. Two carboxy-terminal mutati

SUBMITTER: El Mouali Y 

PROVIDER: S-EPMC8594473 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature