Thermal Stability Changes in Telomeric G-Quadruplex Structures Due to N⁶-Methyladenine Modification.

ABSTRACT: N⁶-methyladenine modification (m⁶dA) has recently been identified in eukaryote genomic DNA. The methylation destabilizes the duplex structure when the adenine forms a Watson-Crick base pair, whereas the methylation on a terminal unpaired adenine stabilizes the duplex structure by increasing the stacking interaction. In this study, the effects of m⁶dA modification on the thermal stability of four distinct telomeric G-quadruplex (G4) structures were investigated. The m⁶dA-modified telomeric oligonucleotide d[AGGG(TTAGGG)₃] that forms a basket-type G4 in Na⁺, d[(TTAGGG)₄TT] that forms a hybrid-type G4 in K⁺ (Form-2), d[AAAGGG(TTAGGG)₃AA] that forms a hybrid-type G4 in K⁺ (Form-

SUBMITTER: Wada R 

PROVIDER: S-EPMC8594671 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature