Deciphering Plant Chromatin Regulation via CRISPR/dCas9-Based Epigenome Engineering.

ABSTRACT: CRISPR-based epigenome editing uses dCas9 as a platform to recruit transcription or chromatin regulators at chosen loci. Despite recent and ongoing advances, the full potential of these approaches to studying chromatin functions in vivo remains challenging to exploit. In this review we discuss how recent progress in plants and animals provides new routes to investigate the function of chromatin regulators and address the complexity of associated regulations that are often interconnected. While efficient transcriptional engineering methodologies have been developed and can be used as tools to alter the chromatin state of a locus, examples of direct manipulation of chromatin regulators remain scarce in plants. These reports also reveal pitfalls and limitations of epigenome engineering approa

SUBMITTER: Dubois A 

PROVIDER: S-EPMC8594717 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature