Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth.

ABSTRACT: Regulation of growth and cell size is crucial for the optimization of bacterial cellular function. So far, single bacterial cells have been found to grow predominantly exponentially, which implies the need for tight regulation to maintain cell size homeostasis. Here, we characterize the growth behavior of the apically growing bacterium Corynebacterium glutamicum using a novel broadly applicable inference method for single-cell growth dynamics. Using this approach, we find that C. glutamicum exhibits asymptotically linear single-cell growth. To explain this growth mode, we model elongation as being rate-limited by the apical growth mechanism. Our model accurately reproduces the inferred cell growth dynamics and is validated with elongation measurements on a transglycosylase de

SUBMITTER: Messelink JJ 

PROVIDER: S-EPMC8594916 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature