Enhancing diversity analysis by repeatedly rarefying next generation sequencing data describing microbial communities.

ABSTRACT: Amplicon sequencing has revolutionized our ability to study DNA collected from environmental samples by providing a rapid and sensitive technique for microbial community analysis that eliminates the challenges associated with lab cultivation and taxonomic identification through microscopy. In water resources management, it can be especially useful to evaluate ecosystem shifts in response to natural and anthropogenic landscape disturbances to signal potential water quality concerns, such as the detection of toxic cyanobacteria or pathogenic bacteria. Amplicon sequencing data consist of discrete counts of sequence reads, the sum of which is the library size. Groups of samples typically have different library sizes that are not representative of biological variation; library size normalizatio

SUBMITTER: Cameron ES 

PROVIDER: S-EPMC8595385 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature