Probing multiple enzymatic methylation events in real time with NMR spectroscopy.

ABSTRACT: Post-translational modification (PTM) of proteins is of critical importance to the regulation of many cellular processes in eukaryotic organisms. One of the most well-studied protein PTMs is methylation, wherein an enzyme catalyzes the transfer of a methyl group from a cofactor to a lysine or arginine side chain. Lysine methylation is especially abundant in the histone tails and is an important marker for denoting active or repressed genes. Given their relevance to transcriptional regulation, the study of methyltransferase function through in vitro experiments is an important stepping stone toward understanding the complex mechanisms of regulated gene expression. To date, most methyltransferase characterization strategies rely on the use of radioactive cofactors, detection of a methyl tran

SUBMITTER: Usher ET 

PROVIDER: S-EPMC8595733 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature