Strategies for mass spectrometry-based phosphoproteomics using isobaric tagging.

ABSTRACT:

Introduction

Protein phosphorylation is a primary mechanism of signal transduction in cellular systems. Isobaric tagging can be used to investigate alterations in phosphorylation events in sample multiplexing experiments where quantification extends across all conditions. As such, innovations in tandem mass tag methods can facilitate the expansion of the depth and breadth of phosphoproteomic analyses.

Areas covered

This review discusses the current state of tandem mass tag-centric phosphoproteomics and highlights advances in reagent chemistry, instrumentation, data acquisition, and data analysis. We stress that approaches for phosphoproteomic investigations require high-specificity enrichment, sensitive detection, and accurate phosphorylation site localization.

Expert opi

SUBMITTER: Liu X 

PROVIDER: S-EPMC8595857 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature