Probing Multicellular Tissue Fusion of Cocultured Spheroids-A 3D-Bioassembly Model.

ABSTRACT: While decades of research have enriched the knowledge of how to grow cells into mature tissues, little is yet known about the next phase: fusing of these engineered tissues into larger functional structures. The specific effect of multicellular interfaces on tissue fusion remains largely unexplored. Here, a facile 3D-bioassembly platform is introduced to primarily study fusion of cartilage-cartilage interfaces using spheroids formed from human mesenchymal stromal cells (hMSCs) and articular chondrocytes (hACs). 3D-bioassembly of two adjacent hMSCs spheroids displays coordinated migration and noteworthy matrix deposition while the interface between two hAC tissues lacks both cells and type-II collagen. Cocultures contribute to increased phenotypic stability in the fusion region while close

SUBMITTER: Lindberg GCJ 

PROVIDER: S-EPMC8596109 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature