Singlet-Oxygen-Induced Phospholipase A₂ Inhibition: A Major Role for Interfacial Tryptophan Dioxidation.

ABSTRACT: Several studies have revealed that various diseases such as cancer have been associated with elevated phospholipase A₂ (PLA₂ ) activity. Therefore, the regulation of PLA₂ catalytic activity is undoubtedly vital. In this study, effective inactivation of PLA₂ due to reactive species produced from cold physical plasma as a source to model oxidative stress is reported. We found singlet oxygen to be the most relevant active agent in PLA₂ inhibition. A more detailed analysis of the plasma-treated PLA₂ identified tryptophan 128 as a hot spot, rich in double oxidation. The significant dioxidation of this interfacial tryptophan resulted in an N-formylkynurenine product via the oxidative opening of the tryptophan indole ring. Molecula

SUBMITTER: Nasri Z 

PROVIDER: S-EPMC8596696 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature