Dead or alive: microbial viability treatment reveals both active and inactive bacterial constituents in the fish gut microbiota.

ABSTRACT:

Aims

This study evaluated the microbial viability of fish gut microbiota in both digesta (faecal) and mucosal samples using a modified propidium monoazide (PMA) protocol, followed by 16S ribosomal RNA (rRNA) gene sequencing.

Methods and results

Digesta and gut mucosal samples from farmed yellowtail kingfish (Seriola lalandi) were collected and a modified PMA treatment was applied prior to DNA extraction to differentiate both active and nonviable microbial cells in the samples. All samples were then sequenced using a standard 16S rRNA approach. The digesta and mucosal samples contained significantly different bacterial communities, with a higher diversity observed in digesta samples. In addition, PMA treatment significantly reduced the microbial diversity and richness of dige

SUBMITTER: Legrand TPRA 

PROVIDER: S-EPMC8596808 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature