Evaluation validation of a qPCR curve analysis method and conventional approaches.

ABSTRACT:

Background

Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) is a sensitive and reliable method for mRNA quantification and rapid analysis of gene expression from a large number of starting templates. It is based on the statistical significance of the beginning of exponential phase in real-time PCR kinetics, reflecting quantitative cycle of the initial target quantity and the efficiency of the PCR reaction (the fold increase of product per cycle).

Results

We used the large clinical biomarker dataset and 94-replicates-4-dilutions set which was published previously as research tools, then proposed a new qPCR curve analysis method--C_qMAN, to determine the position of quantitative cycle as well as the efficiency of the PCR reaction and applied in

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8596907 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature