Chemoselective and Highly Sensitive Quantification of Gut Microbiome and Human Metabolites.

ABSTRACT: The microbiome has a fundamental impact on the human host's physiology through the production of highly reactive compounds that can lead to disease development. One class of such compounds are carbonyl-containing metabolites, which are involved in diverse biochemical processes. Mass spectrometry is the method of choice for analysis of metabolites but carbonyls are analytically challenging. Herein, we have developed a new chemical biology tool using chemoselective modification to overcome analytical limitations. Two isotopic probes allow for the simultaneous and semi-quantitative analysis at the femtomole level as well as qualitative analysis at attomole quantities that allows for detection of more than 200 metabolites in human fecal, urine and plasma samples. This comprehensive mass spectr

SUBMITTER: Lin W 

PROVIDER: S-EPMC8597006 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature