CoRe: a robustly benchmarked R package for identifying core-fitness genes in genome-wide pooled CRISPR-Cas9 screens.

ABSTRACT:

Background

CRISPR-Cas9 genome-wide screens are being increasingly performed, allowing systematic explorations of cancer dependencies at unprecedented accuracy and scale. One of the major computational challenges when analysing data derived from such screens is to identify genes that are essential for cell survival invariantly across tissues, conditions, and genomic-contexts (core-fitness genes), and to distinguish them from context-specific essential genes. This is of paramount importance to assess the safety profile of candidate therapeutic targets and for elucidating mechanisms involved in tissue-specific genetic diseases.

Results

We have developed CoRe: an R package implementing existing and novel methods for the identification of core-fitness genes (at two different leve

SUBMITTER: Vinceti A 

PROVIDER: S-EPMC8597285 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature