Differential uptake of three clinically relevant allergens by human plasmacytoid dendritic cells.

ABSTRACT:

Background

Human plasmacytoid dendritic cells (pDC) have a dual role as interferon-producing and antigen-presenting cells. Their relevance for allergic diseases is controversial. and the impact of pDC on allergic immune responses is poorly understood.

Methods

This in vitro study on human pDC isolated from peripheral blood was designed to compare side by side the uptake of three clinically relevant representative allergens: fluorochrome-labeled house dust mite Der p 1, Bee venom extract from Apis mellifera (Api) and the food allergen OVA analyzed flow cytometry and confocal microscopy.

Results

We found that the internalization and its regulation by TLR9 ligation was significantly different between allergens in terms of time course and strength of uptake. Api and OVA up

SUBMITTER: Zurmuhl N 

PROVIDER: S-EPMC8597288 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature