Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells.

ABSTRACT:

Background

The Mediator complex is an evolutionarily conserved multi-subunit protein complex that plays major roles in transcriptional activation and is essential for cell growth, proliferation, and differentiation. Recent studies revealed that some Mediator subunits formed nuclear condensates that may facilitate enhancer-promoter interactions and gene activation. The assembly, regulation, and functions of these nuclear condensates remain to be further understood.

Results

We found that Med15, a subunit in the tail module of the Mediator complex, formed nuclear condensates through a novel mechanism. Nuclear foci of Med15 were detected by both immunostaining of endogenous proteins and live cell imaging. Like Med1 foci and many other biomolecular condensates, Med15 foci were se

SUBMITTER: Shi Y 

PROVIDER: S-EPMC8597291 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature