FISH Going Meso-Scale: A Microscopic Search for Chromatin Domains.

ABSTRACT: The intimate relationships between genome structure and function direct efforts toward deciphering three-dimensional chromatin organization within the interphase nuclei at different genomic length scales. For decades, major insights into chromatin structure at the level of large-scale euchromatin and heterochromatin compartments, chromosome territories, and subchromosomal regions resulted from the evolution of light microscopy and fluorescence in situ hybridization. Studies of nanoscale nucleosomal chromatin organization benefited from a variety of electron microscopy techniques. Recent breakthroughs in the investigation of mesoscale chromatin structures have emerged from chromatin conformation capture methods (C-methods). Chromatin has been found to form hierarchical domains with h

SUBMITTER: Maslova A 

PROVIDER: S-EPMC8597843 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature