Tracking the ATP-binding response in adenylate kinase in real time.

ABSTRACT: The biological function of proteins is critically dependent on dynamics inherent to the native structure. Such structural dynamics obey a predefined order and temporal timing to execute the specific reaction. Determination of the cooperativity of key structural rearrangements requires monitoring protein reactions in real time. In this work, we used time-resolved x-ray solution scattering (TR-XSS) to visualize structural changes in the Escherichia coli adenylate kinase (AdK) enzyme upon laser-induced activation of a protected ATP substrate. A 4.3-ms transient intermediate showed partial closing of both the ATP- and AMP-binding domains, which indicates a cooperative closing mechanism. The ATP-binding domain also showed local unfolding and breaking of an Arg¹³¹-Asp^146</

SUBMITTER: Oradd F 

PROVIDER: S-EPMC8597995 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature