Reduced Physical Activity Alters the Leucine-Stimulated Translatome in Aged Skeletal Muscle.

ABSTRACT: Periods of inactivity experienced by older adults induce nutrient anabolic resistance creating a cascade of skeletal muscle transcriptional and translational aberrations contributing to muscle dysfunction. The purpose of this study was to identify how inactivity alters leucine-stimulated translation of molecules and pathways within the skeletal muscle of older adults. We performed ribosomal profiling alongside RNA sequencing from skeletal muscle biopsies taken from older adults (n = 8; ~72 years; 6 F/2 M) in response to a leucine bolus before (Active) and after (Reduced Activity) 2 weeks of reduced physical activity. At both visits, muscle biopsies were taken at baseline, 60 minutes (early response), and 180 minutes (late response) after leucine ingestion. Previously identified inactivity-

SUBMITTER: Mahmassani ZS 

PROVIDER: S-EPMC8599012 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature