Beyond Ca²⁺ signalling: the role of TRPV3 in the transport of NH₄.

ABSTRACT: Mutations of TRPV3 lead to severe dermal hyperkeratosis in Olmsted syndrome, but whether the mutants are trafficked to the cell membrane or not is controversial. Even less is known about TRPV3 function in intestinal epithelia, although research on ruminants and pigs suggests an involvement in the uptake of NH₄⁺. It was the purpose of this study to measure the permeability of the human homologue (hTRPV3) to NH₄⁺, to localize hTRPV3 in human skin equivalents, and to investigate trafficking of the Olmsted mutant G573S. Immunoblotting and immunostaining verified the successful expression of hTRPV3 in HEK-293 cells and Xenopus oocytes with trafficking to the cell membrane. Human skin equivalents showed distinct staining of the apical membrane of the t

SUBMITTER: Liebe H 

PROVIDER: S-EPMC8599221 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature