Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector delivery.

ABSTRACT: Prime editing is a recent precision genome editing modality whose versatility offers the prospect for a wide range of applications, including the development of targeted genetic therapies. Yet, an outstanding bottleneck for its optimization and use concerns the difficulty in delivering large prime editing complexes into cells. Here, we demonstrate that packaging prime editing constructs in adenoviral capsids overcomes this constrain resulting in robust genome editing in both transformed and non-transformed human cells with up to 90% efficiencies. Using this cell cycle-independent delivery platform, we found a direct correlation between prime editing activity and cellular replication and disclose that the proportions between accurate prime editing events and unwanted byproducts can be influ

SUBMITTER: Wang Q 

PROVIDER: S-EPMC8599732 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature