Unveiling mRNP composition by fluorescence correlation and cross-correlation spectroscopy using cell lysates.

ABSTRACT: Understanding the mRNA life cycle requires information about the dynamics and macromolecular composition and stoichiometry of mRNPs. Fluorescence correlation and cross-correlation spectroscopy (FCS and FCCS) are appealing technologies to study these macromolecular structures because they have single molecule sensitivity and readily provide information about their molecular composition and dynamics. Here, we demonstrate how FCS can be exploited to study cytoplasmic mRNPs with high accuracy and reproducibility in cell lysates. Cellular lysates not only recapitulate data from live cells but provide improved readings and allow investigation of single mRNP analysis under particular conditions or following enzymatic treatments. Moreover, FCCS employing minute amounts of cells closely corroborate

SUBMITTER: Mateu-Regue A 

PROVIDER: S-EPMC8599746 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature