Bisecting Lewis X in Hybrid-Type <i>N</i>-Glycans of Human Brain Revealed by Deep Structural Glycomics.

ABSTRACT: The importance of protein glycosylation in the biomedical field requires methods that not only quantitate structures by their monosaccharide composition, but also resolve and identify the many isomers expressed by mammalian cells. The art of unambiguous identification of isomeric structures in complex mixtures, however, did not yet catch up with the fast pace of advance of high-throughput glycomics. Here, we present a strategy for deducing structures with the help of a deci-minute accurate retention time library for porous graphitic carbon chromatography with mass spectrometric detection. We implemented the concept for the fundamental N-glycan type consisting of five hexoses, four N-acetylhexosamines and one fucose residue. Nearly all of the 40 biosynthetized isomers occupied

SUBMITTER: Helm J 

PROVIDER: S-EPMC8600501 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature