Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10.

ABSTRACT:

Background

This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC.

Methods

In this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors.

Results

We found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 w

SUBMITTER: Li W 

PROVIDER: S-EPMC8600898 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature