DNA damage checkpoint activation affects peptidoglycan synthesis and late divisome components in Bacillus subtilis.

ABSTRACT: During normal DNA replication, all cells encounter damage to their genetic material. As a result, organisms have developed response pathways that provide time for the cell to complete DNA repair before cell division occurs. In Bacillus subtilis, it is well established that the SOS-induced cell division inhibitor YneA blocks cell division after genotoxic stress; however, it remains unclear how YneA enforces the checkpoint. Here, we identify mutations that disrupt YneA activity and mutations that are refractory to the YneA-induced checkpoint. We find that YneA C-terminal truncation mutants and point mutants in or near the LysM peptidoglycan binding domain render YneA incapable of checkpoint enforcement. In addition, we develop a genetic method which isolated mutations in the ftsW gene that c

SUBMITTER: Masser EA 

PROVIDER: S-EPMC8600973 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature