Collision energy-breakdown curves - An additional tool to characterize MS/MS methods.

ABSTRACT:

Background

In tandem mass spectrometry, analyte detection is based on collision-induced fragmentation, which is modulated by the collision energy (CE) setting. Variation in CE leads to differential ion yield, and optimization is usually performed empirically as "tuning" during method development. Our aim was to build a method to objectify the impact of collision energy settings on ion yield for individual compounds.

Methods

Collision energy (CE)-breakdown curves were generated based on acquisition files in which a large number of quasi-identical mass transitions were recorded simultaneously, with variation of CE over a defined range within a single injection. Ion yield (normalized to an internal standard recorded with a locked collision energy) was plotted as a curve versus

SUBMITTER: Morlein S 

PROVIDER: S-EPMC8600984 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature