An isotope dilution LC-MS/MS-based candidate reference method for the quantification of androstenedione in human serum and plasma.

ABSTRACT: The accurate measurement of androstenedione in human serum and plasma is required for steroid profiling to assure the appropriate diagnosis and differential diagnosis of hyperandrogenism. In this work, we introduce an isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) candidate reference measurement procedure for the quantification of androstenedione in human serum and plasma. The performance of the procedure enables its use in the evaluation and standardization of routine assays and for the evaluation of patient samples to ensure the traceability of individual patient results. As the primary standard, a certified reference material from NMIA (National Measurement Institute, Australia) was used. Additionally, a quantitative nuclear magnetic resonance (qNMR) method w

SUBMITTER: Gradl K 

PROVIDER: S-EPMC8600989 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature