Yarrowia lipolytica chassis strains engineered to produce aromatic amino acids via the shikimate pathway.

ABSTRACT: Yarrowia lipolytica is widely used as a microbial producer of lipids and lipid derivatives. Here, we exploited this yeast's potential to generate aromatic amino acids by developing chassis strains optimized for the production of phenylalanine, tyrosine and tryptophan. We engineered the shikimate pathway to overexpress a combination of Y. lipolytica and heterologous feedback-insensitive enzyme variants. Our best chassis strain displayed high levels of de novo Ehrlich metabolite production (up to 0.14 g l^-1 in minimal growth medium), which represented a 93-fold increase compared to the wild-type strain (0.0015 g l^-1 ). Production was further boosted to 0.48 g l^-1 when glycerol, a low-cost carbon source, was used, concomitantly to high secretion of phenylalani

SUBMITTER: Larroude M 

PROVIDER: S-EPMC8601196 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature