High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica.

ABSTRACT: The natural plant product bisabolene serves as a precursor for the production of a wide range of industrially relevant chemicals. However, the low abundance of bisabolene in plants renders its isolation from plant sources non-economically viable. Therefore, creation of microbial cell factories for bisabolene production supported by synthetic biology and metabolic engineering strategies presents a more competitive and environmentally sustainable method for industrial production of bisabolene. In this proof-of-principle study, for the first time, we engineered the oleaginous yeast Yarrowia lipolytica to produce α-bisabolene, β-bisabolene and γ-bisabolene through heterologous expression of the α-bisabolene synthase from Abies grandis, the β-bisabolene synthase gene from Zingiber officinale an

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8601197 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature