Bacterial Swarmers Enriched During Intestinal Stress Ameliorate Damage.

ABSTRACT:

Background and aims

Bacterial swarming, a collective movement on a surface, has rarely been associated with human pathophysiology. This study aims to define a role for bacterial swarmers in amelioration of intestinal stress.

Methods

We developed a polymicrobial plate agar assay to detect swarming and screened mice and humans with intestinal stress and inflammation. From chemically induced colitis in mice, as well as humans with inflammatory bowel disease, we developed techniques to isolate the dominant swarmers. We developed swarm-deficient but growth and swim-competent mutant bacteria as isogenic controls. We performed bacterial reinoculation studies in mice with colitis, fecal 16S, and meta-transcriptomic analyses, as well as in vitro microbial interaction studies.

Resu

SUBMITTER: De A 

PROVIDER: S-EPMC8601393 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature