The effect of soil sample size, for practical DNA extraction, on soil microbial diversity in different taxonomic ranks.

ABSTRACT: To determine the optimal soil sample size for microbial community structure analysis, DNA extraction, microbial composition analysis, and diversity assessments were performed using soil sample sizes of 0.2, 1, and 5 g. This study focused on the relationship between soil amount and DNA extraction container volume and the alteration in microbial composition at different taxonomic ranks (order, class, and phylum). Horizontal (0.2 and 1 g) and vertical (5 g) shaking were applied during DNA extraction for practical use in a small laboratory. In the case of the 5 g soil sample, DNA extraction efficiency and the value of α-diversity index fluctuated severely, possibly because of vertical shaking. Regarding the 0.2 and 1 g soil samples, the number of taxa, Shannon-Wiener index, and Bray-Curtis dis

SUBMITTER: Morita H 

PROVIDER: S-EPMC8601499 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature