Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts.

ABSTRACT: It has been argued that DNA repair by homologous recombination in the context of endonuclease-mediated cleavage can cause mutations. To better understand this phenomenon, we examined homologous recombination following endonuclease cleavage in a native genomic context: the movement of self-splicing introns in the mitochondrial genomes of Metschnikowia yeasts. Self-splicing mitochondrial introns are mobile elements, which can copy and paste themselves at specific insertion sites in mitochondrial DNA using a homing endonuclease in conjunction with homologous recombination. Here, we explore the mutational effects of self-splicing introns by comparing sequence variation within the intron-rich cox1 and cob genes from 71 strains (belonging to 40 species) from the yeast genus

SUBMITTER: Thompson SRL 

PROVIDER: S-EPMC8601654 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature