The molecular and phenotypic makeup of fetal human skin T lymphocytes.

ABSTRACT: The adult human skin contains a vast number of T cells that are essential for skin homeostasis and pathogen defense. T cells are first observed in the skin at the early stages of gestation; however, our understanding of their contribution to early immunity has been limited by their low abundance and lack of comprehensive methodologies for their assessment. Here, we describe a new workflow for isolating and expanding significant amounts of T cells from fetal human skin. Using multiparametric flow cytometry and in situ immunofluorescence, we found a large population with a naive phenotype and small populations with a memory and regulatory phenotype. Their molecular state was characterized using single-cell transcriptomics and TCR repertoire profiling. Importantly, culture of total fetal skin

SUBMITTER: Reitermaier R 

PROVIDER: S-EPMC8601710 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature