Complementing the pulp proteome via sampling with a picosecond infrared laser (PIRL).

ABSTRACT:

Objectives

The aim of this investigation was the detailed analysis of the human pulp proteome using the new picosecond infrared laser (PIRL)-based sampling technique, which is based on a completely different mechanism compared to mechanical sampling. Proteome analysis of healthy pulp can provide data to define changes in the proteome associated with dental disease.

Material and methods

Immediately after extraction of the entire, undamaged tooth, 15 wisdom teeth were deep frozen in liquid nitrogen and preserved at -80°C. Teeth were crushed, and the excised frozen pulps were conditioned for further analysis. The pulps were sampled using PIRL, and the aspirates digested with trypsin and analyzed with mass spectrometry. Pulp proteins were categorized according to their gene onto

SUBMITTER: Feridouni Khamaneh Y 

PROVIDER: S-EPMC8602158 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature