Follicular T cells are clonally and transcriptionally distinct in B cell-driven mouse autoimmune disease.

ABSTRACT: Pathogenic autoantibodies contribute to tissue damage and clinical decline in autoimmune disease. Follicular T cells are central regulators of germinal centers, although their contribution to autoantibody-mediated disease remains unclear. Here we perform single cell RNA and T cell receptor (TCR) sequencing of follicular T cells in a mouse model of autoantibody-mediated disease, allowing for analyses of paired transcriptomes and unbiased TCRαβ repertoires at single cell resolution. A minority of clonotypes are preferentially shared amongst autoimmune follicular T cells and clonotypic expansion is associated with differential gene signatures in autoimmune disease. Antigen prediction using algorithmic and machine learning approaches indicates convergence towards shared specificities between n

SUBMITTER: Akama-Garren EH 

PROVIDER: S-EPMC8602266 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature