G3BP1 inhibits Cul3^SPOP to amplify AR signaling and promote prostate cancer.

ABSTRACT: SPOP, an E3 ubiquitin ligase, acts as a prostate-specific tumor suppressor with several key substrates mediating oncogenic function. However, the mechanisms underlying SPOP regulation are largely unknown. Here, we have identified G3BP1 as an interactor of SPOP and functions as a competitive inhibitor of Cul3^SPOP, suggesting a distinctive mode of Cul3^SPOP inactivation in prostate cancer (PCa). Transcriptomic analysis and functional studies reveal a G3BP1-SPOP ubiquitin signaling axis that promotes PCa progression through activating AR signaling. Moreover, AR directly upregulates G3BP1 transcription to further amplify G3BP1-SPOP signaling in a feed-forward manner. Our study supports a fundamental role of G3BP1 in disabling the tumor suppressive Cul3^SPOP, thus

SUBMITTER: Mukhopadhyay C 

PROVIDER: S-EPMC8602290 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature