Mapping the dynamic transfer functions of eukaryotic gene regulation.

ABSTRACT: Biological information can be encoded within the dynamics of signaling components, which has been implicated in a broad range of physiological processes including stress response, oncogenesis, and stem cell differentiation. To study the complexity of information transfer across the eukaryotic promoter, we screened 119 dynamic conditions-modulating the pulse frequency, amplitude, and pulse width of light-regulating the binding of an epigenome editor to a fluorescent reporter. This system revealed tunable gene expression and filtering behaviors and provided a quantification of the limit to the amount of information that can be reliably transferred across a single promoter as ∼1.7 bits. Using a library of over 100 orthogonal chromatin regulators, we further determined that chromatin state cou

SUBMITTER: Lee JB 

PROVIDER: S-EPMC8602734 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature