Super-Resolution Microscopy Reveals That Stromal Interaction Molecule 1 Trafficking Depends on Microtubule Dynamics.

ABSTRACT: Store-operated Ca²⁺ entry (SOCE) is an essential pathway for Ca²⁺ signaling, and regulates various vital cellular functions. It is triggered by the endoplasmic reticulum Ca²⁺ sensor stromal interaction molecule 1 (STIM1). Illustration of STIM1 spatiotemporal structure at the nanometer scale during SOCE activation provides structural and functional insights into the fundamental Ca²⁺ homeostasis. In this study, we used direct stochastic optical reconstruction microscopy (dSTORM) to revisit the dynamic process of the interaction between STIM1, end-binding protein (EB), and microtubules to the ER-plasma membrane. Using dSTORM, we found that"powder-like"STIM1 aggregates into "trabecular-like" architectures toward the cell periphery during SOCE, and th

SUBMITTER: Huang YT 

PROVIDER: S-EPMC8602801 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature