Exploring the application and mechanism of sodium hyaluronate in cryopreservation of red blood cells.

ABSTRACT: The cryopreservation of red blood cells (RBCs) is essential for transfusion therapy and maintaining the inventory of RBCs units. The existing cryoprotectants (CPAs) have many defects, and the search for novel CPAs is becoming a research hotspot. Sodium hyaluronate (SH) is polymerized from sodium glucuronate and N-acetylglucosamine, which has good water binding capacity and biocompatibility. Herein, we reported for the first time that under the action of medium molecular weight sodium hyaluronate (MSH), the thawed RBCs recovery increased from 33.1 ​± ​5.8% to 63.2 ​± ​3.5%. In addition, RBCs functions and properties were maintained normally, and the residual MSH could be removed by direct washing. When MSH was used with a very low concentration (5% v/v) of glycerol (Gly), the thawed

SUBMITTER: Liu X 

PROVIDER: S-EPMC8603211 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature