Monocyte dysfunction in decompensated cirrhosis is mediated by the prostaglandin E2-EP4 pathway.

ABSTRACT:

Background & aims

Infection is a major problem in advanced liver disease secondary to monocyte dysfunction. Elevated prostaglandin (PG)E₂ is a mediator of monocyte dysfunction in cirrhosis; thus, we examined PGE₂ signalling in outpatients with ascites and in patients hospitalised with acute decompensation to identify potential therapeutic targets aimed at improving monocyte dysfunction.

Methods

Using samples from 11 outpatients with ascites and 28 patients hospitalised with decompensated cirrhosis, we assayed plasma levels of PGE₂ and lipopolysaccharide (LPS); performed quantitative real-time PCR on monocytes; and examined peripheral blood monocyte function. We performed western blotting and immunohistochemistry for PG biosynthetic machinery

SUBMITTER: Maini AA 

PROVIDER: S-EPMC8603213 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature