Immune reactivity and host modulatory roles of two novel Haemonchus contortus cathepsin B-like proteases.

ABSTRACT:

Background

Haemonchus contortus is a blood-feeding, gastrointestinal nematode (GIN) that causes significant economic losses to the small ruminant industry worldwide. Despite extensive efforts, our understanding of the molecular mechanisms used by GIN to evade host immune responses is limited. Cathepsin B-like proteins (CBPs) are members of the cysteine protease family and are involved in parasite invasion and thus provide viable vaccine candidates.

Methods

In silico comparative analysis was used to identify conserved proteins among a subset of clade V parasitic nematodes with emphasis on blood-feeding worms, among which CBPs appeared prominently. We identified and characterized two novel CBPs designated Hc-CBP-1 and Hc-CBP-2. Rabbit anti-recombinant (r) Hc-CBP-1 and rHc-CBP-

SUBMITTER: Bakshi M 

PROVIDER: S-EPMC8603344 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature