An Optogenetic Tool to Raise Intracellular pH in Single Cells and Drive Localized Membrane Dynamics.

ABSTRACT: Intracellular pH (pHi) dynamics are critical for regulating normal cell physiology. For example, transient increases in pHi (7.2-7.6) regulate cell behaviors like cell polarization, actin cytoskeleton remodeling, and cell migration. Most studies on pH-dependent cell behaviors have been performed at the population level and use nonspecific methods to manipulate pHi. The lack of tools to specifically manipulate pHi at the single-cell level has hindered investigation of the role of pHi dynamics in driving single cell behaviors. In this work, we show that Archaerhodopsin (ArchT), a light-driven outward proton pump, can be used to elicit robust and physiological pHi increases over the minutes time scale. We show that activation of ArchT is repeatable, enabling the maintenance of high pHi in sin

SUBMITTER: Donahue CET 

PROVIDER: S-EPMC8603357 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature