Early inflammatory events of mastitis-a pilot study with the isolated perfused bovine udder.

ABSTRACT:

Background

Bovine mastitis is an important health and cost factor in the milk industry. To elucidate whether isolated perfused bovine udders can be used to study early inflammatory events of mastitis, 1 mg of lipopolysaccharide (LPS) was instilled into quarters of 10 isolated perfused bovine udders. Three hours and 6 h after LPS instillation, tissue samples were taken from the gland cistern and base of the udder, subsequently stored in RNAlater and processed for the determination of inflammation-dependent gene regulation by real-time RT-qPCR. Gene expression analysis was performed using delta-delta Ct method. To translate mRNA results to protein, IL-1ß and IL-6 were determined in tissue homogenate by ELISA.

Results

The instillation of 1 mg LPS lead to an increased expression

SUBMITTER: Brand KS 

PROVIDER: S-EPMC8603483 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature