Identification of a Novel Pyruvyltransferase Using ¹³C Solid-State Nuclear Magnetic Resonance To Analyze Rhizobial Exopolysaccharides.

ABSTRACT: The alphaproteobacterium Sinorhizobium meliloti secretes two acidic exopolysaccharides (EPSs), succinoglycan (EPSI) and galactoglucan (EPSII), which differentially enable it to adapt to a changing environment. Succinoglycan is essential for invasion of plant hosts and, thus, for the formation of nitrogen-fixing root nodules. Galactoglucan is critical for population-based behaviors such as swarming and biofilm formation and can facilitate invasion in the absence of succinoglycan on some host plants. The biosynthesis of galactoglucan is not as completely understood as that of succinoglycan. We devised a pipeline to identify putative pyruvyltransferase and acetyltransferase genes, construct genomic deletions in strains engineered to produce either succinoglycan or galactoglucan, and analyze E

SUBMITTER: Wells DH 

PROVIDER: S-EPMC8604072 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature