Identification of nocardia species by restriction endonuclease analysis of an amplified portion of the 16S rRNA gene.

ABSTRACT: Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuclease analysis (REA) for species identification. Unique restriction fragment length polymorphism (RFLP) patterns were found for Nocardia sp. type strains (except for the N. asteroides type strain) and representative isolates of the drug pattern types of Nocardia asteroides (except for N. asteroides drug pattern type IV, which gave inconsistent amplification). A variant RFLP pattern for Nocardia nova was also observed. Twenty-eight clinical isolates were evaluated both by tradi

SUBMITTER: Conville PS 

PROVIDER: S-EPMC86045 | biostudies-literature | 2000 Jan

REPOSITORIES: biostudies-literature