Proteome and functional decline as platelets age in the circulation.

ABSTRACT:

Background

Platelets circulate in the blood of healthy individuals for approximately 7-10 days regulated by finely balanced processes of production and destruction. As platelets are anucleate we reasoned that their protein composition would change as they age and that this change would be linked to alterations in structure and function.

Objective

To isolate platelets of different ages from healthy individuals to test the hypothesis that changes in protein content cause alterations in platelet structure and function.

Methods

Platelets were separated according to thiazole orange fluorescence intensity as a surrogate indicator of mRNA content and so a marker of platelet age and then subjected to proteomics, imaging, and functional assays to produce an in-depth analysis o

SUBMITTER: Allan HE 

PROVIDER: S-EPMC8604765 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature