Improved Stability of Engineered Ammonia Production in the Plant-Symbiont <i>Azospirillum brasilense</i>.

ABSTRACT: Bioavailable nitrogen is the limiting nutrient for most agricultural food production. Associative diazotrophs can colonize crop roots and fix their own bioavailable nitrogen from the atmosphere. Wild-type (WT) associative diazotrophs, however, do not release fixed nitrogen in culture and are not known to directly transfer fixed nitrogen resources to plants. Efforts to engineer diazotrophs for plant nitrogen provision as an alternative to chemical fertilization have yielded several strains that transiently release ammonia. However, these strains suffer from selection pressure for nonproducers, which rapidly deplete ammonia accumulating in culture, likely limiting their potential for plant growth promotion (PGP). Here we report engineered Azospirillum brasilense strains with significa

SUBMITTER: Schnabel T 

PROVIDER: S-EPMC8604774 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature